mouse anti-lacz Search Results


mouse anti-lacz  (Promega)
90
Promega mouse anti-lacz
Mouse Anti Lacz, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-lacz/mouse+anti+lacz/10__2139_slash_ssrn__4076344-217-28-30
Average 90 stars, based on 1 article reviews
mouse anti-lacz - by Bioz Stars, 2026-11
90/100 stars
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mouse anti-lacz  (ICN Pharmaceuticals)
90
ICN Pharmaceuticals mouse anti-lacz
Tpit is required for <t>late</t> <t>POMC</t> lineage differentiation but not for lineage commitment. Immunohistochemistry showing almost complete disappearance of pituitary POMC-expressing cells in Tpit−/− mice compared to heterozygotes (+/−) or wild-type (+/+, not shown), in both E14.5 embryo (A) and adult (E). No difference was observed between +/+ and +/− mice throughout these analyses. (B) Immunohistochemistry showing normal distribution of NeuroD1 expression in E14.5 Tpit−/− pituitary. (C) Immunohistochemistry showing <t>lacZ</t> expression in Tpit−/− E14.5 pituitary. Distribution of lacZ-positive cells is similar to normal POMC and NeuroD1 expression at this stage of development. (D) X-gal staining showing lacZ expression in both Tpit+/− and Tpit−/− adult pituitaries. In Tpit−/− pituitary, a few positive cells are present in anterior lobe (AL), and intermediate lobe (IL) is hypoplastic, with all lacZ-positive cells. (E) POMC staining reveals a few POMC-positive cells in AL and IL.
Mouse Anti Lacz, supplied by ICN Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-lacz/mouse+anti+lacz/pmc00196016-378-19-22
Average 90 stars, based on 1 article reviews
mouse anti-lacz - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
mouse anti-lacz promega  (Promega)
90
Promega mouse anti-lacz promega
Tpit is required for <t>late</t> <t>POMC</t> lineage differentiation but not for lineage commitment. Immunohistochemistry showing almost complete disappearance of pituitary POMC-expressing cells in Tpit−/− mice compared to heterozygotes (+/−) or wild-type (+/+, not shown), in both E14.5 embryo (A) and adult (E). No difference was observed between +/+ and +/− mice throughout these analyses. (B) Immunohistochemistry showing normal distribution of NeuroD1 expression in E14.5 Tpit−/− pituitary. (C) Immunohistochemistry showing <t>lacZ</t> expression in Tpit−/− E14.5 pituitary. Distribution of lacZ-positive cells is similar to normal POMC and NeuroD1 expression at this stage of development. (D) X-gal staining showing lacZ expression in both Tpit+/− and Tpit−/− adult pituitaries. In Tpit−/− pituitary, a few positive cells are present in anterior lobe (AL), and intermediate lobe (IL) is hypoplastic, with all lacZ-positive cells. (E) POMC staining reveals a few POMC-positive cells in AL and IL.
Mouse Anti Lacz Promega, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-lacz/mouse+anti+lacz+promega/pm12015986-193-7-9
Average 90 stars, based on 1 article reviews
mouse anti-lacz promega - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
mouse anti- lacz monoclonal antibody  (Cusabio)
N/A
Mouse anti-Escherichia coli lacZ Monoclonal Antibody
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Image Search Results


Tpit is required for late POMC lineage differentiation but not for lineage commitment. Immunohistochemistry showing almost complete disappearance of pituitary POMC-expressing cells in Tpit−/− mice compared to heterozygotes (+/−) or wild-type (+/+, not shown), in both E14.5 embryo (A) and adult (E). No difference was observed between +/+ and +/− mice throughout these analyses. (B) Immunohistochemistry showing normal distribution of NeuroD1 expression in E14.5 Tpit−/− pituitary. (C) Immunohistochemistry showing lacZ expression in Tpit−/− E14.5 pituitary. Distribution of lacZ-positive cells is similar to normal POMC and NeuroD1 expression at this stage of development. (D) X-gal staining showing lacZ expression in both Tpit+/− and Tpit−/− adult pituitaries. In Tpit−/− pituitary, a few positive cells are present in anterior lobe (AL), and intermediate lobe (IL) is hypoplastic, with all lacZ-positive cells. (E) POMC staining reveals a few POMC-positive cells in AL and IL.

Journal:

Article Title: Tpit determines alternate fates during pituitary cell differentiation

doi: 10.1101/gad.1065703

Figure Lengend Snippet: Tpit is required for late POMC lineage differentiation but not for lineage commitment. Immunohistochemistry showing almost complete disappearance of pituitary POMC-expressing cells in Tpit−/− mice compared to heterozygotes (+/−) or wild-type (+/+, not shown), in both E14.5 embryo (A) and adult (E). No difference was observed between +/+ and +/− mice throughout these analyses. (B) Immunohistochemistry showing normal distribution of NeuroD1 expression in E14.5 Tpit−/− pituitary. (C) Immunohistochemistry showing lacZ expression in Tpit−/− E14.5 pituitary. Distribution of lacZ-positive cells is similar to normal POMC and NeuroD1 expression at this stage of development. (D) X-gal staining showing lacZ expression in both Tpit+/− and Tpit−/− adult pituitaries. In Tpit−/− pituitary, a few positive cells are present in anterior lobe (AL), and intermediate lobe (IL) is hypoplastic, with all lacZ-positive cells. (E) POMC staining reveals a few POMC-positive cells in AL and IL.

Article Snippet: Antibodies were used as follows: rabbit anti-Tpit 1:200 ( Lamolet et al. 2001 ), mouse anti-POMC 1:500 (Cortex Biochem), mouse anti-lacZ 1:500 (ICN Pharmaceuticals), rabbit anti-SF1 1:1500 (kind gift from K. Morohashi, National Institute for Basic Biology, Okazaki, Japan), rabbit anti-Pit-1 1:50 (Santa Cruz Biotechnology), rabbit anti-αGSU 1:500, rabbit anti-βFSH 1:200, rabbit anti-prolactin 1:1000, rabbit anti-GH 1: 1670, guinea pig anti-βLH 1:200 (all pituitary hormones antibodies were kindly provided by A.F.

Techniques: Immunohistochemistry, Expressing, Staining

Alternate pituitary cell fates in the absence of Tpit. The hypoplastic intermediate lobe (IL, bracketed by arrows) of Tpit−/− pituitaries contains gonadotroph and Pit-1-independent thyrotroph cells in addition to the few POMC-positive cells (Fig. ​(Fig.2E).2E). (A) Gonadotroph cells are revealed using immunohistochemical analysis for αGSU, βLH, βFSH, and SF1. Thyrotrophs are revealed using anti-TSH antibody. Very few TSH-positive clusters were present in all mutant pituitaries examined. Pit-1 immunoreactivity was never detected in hypoplastic IL, including in sections adjacent to TSH-positive cell clusters. PRL and GH were never detected in hypoplastic IL. (B) Colocalization immunohistochemistry indicates that hypoplastic IL cells are either POMC lineage or gonadotroph. Colabeling with POMC and αGSU never showed any cells positive for both. Colocalization between αGSU and βLH showed all βLH cells to be αGSU-positive. (C) Ectopic expression of SF1 in AL of E16.5 Tpit−/− pituitaries. The dotted area represents a cluster of POMC-positive cells usually observed on the ventrocaudal side of normal (+/+) E16.5 pituitaries. A similar pattern of lacZ-positive cells was observed in Tpit−/− pituitary. This area is usually devoid of SF1-positive cells in normal (+/+) pituitaries, but Tpit−/− pituitaries exhibit SF1-positive cells in this area.

Journal:

Article Title: Tpit determines alternate fates during pituitary cell differentiation

doi: 10.1101/gad.1065703

Figure Lengend Snippet: Alternate pituitary cell fates in the absence of Tpit. The hypoplastic intermediate lobe (IL, bracketed by arrows) of Tpit−/− pituitaries contains gonadotroph and Pit-1-independent thyrotroph cells in addition to the few POMC-positive cells (Fig. ​(Fig.2E).2E). (A) Gonadotroph cells are revealed using immunohistochemical analysis for αGSU, βLH, βFSH, and SF1. Thyrotrophs are revealed using anti-TSH antibody. Very few TSH-positive clusters were present in all mutant pituitaries examined. Pit-1 immunoreactivity was never detected in hypoplastic IL, including in sections adjacent to TSH-positive cell clusters. PRL and GH were never detected in hypoplastic IL. (B) Colocalization immunohistochemistry indicates that hypoplastic IL cells are either POMC lineage or gonadotroph. Colabeling with POMC and αGSU never showed any cells positive for both. Colocalization between αGSU and βLH showed all βLH cells to be αGSU-positive. (C) Ectopic expression of SF1 in AL of E16.5 Tpit−/− pituitaries. The dotted area represents a cluster of POMC-positive cells usually observed on the ventrocaudal side of normal (+/+) E16.5 pituitaries. A similar pattern of lacZ-positive cells was observed in Tpit−/− pituitary. This area is usually devoid of SF1-positive cells in normal (+/+) pituitaries, but Tpit−/− pituitaries exhibit SF1-positive cells in this area.

Article Snippet: Antibodies were used as follows: rabbit anti-Tpit 1:200 ( Lamolet et al. 2001 ), mouse anti-POMC 1:500 (Cortex Biochem), mouse anti-lacZ 1:500 (ICN Pharmaceuticals), rabbit anti-SF1 1:1500 (kind gift from K. Morohashi, National Institute for Basic Biology, Okazaki, Japan), rabbit anti-Pit-1 1:50 (Santa Cruz Biotechnology), rabbit anti-αGSU 1:500, rabbit anti-βFSH 1:200, rabbit anti-prolactin 1:1000, rabbit anti-GH 1: 1670, guinea pig anti-βLH 1:200 (all pituitary hormones antibodies were kindly provided by A.F.

Techniques: Immunohistochemical staining, Mutagenesis, Immunohistochemistry, Expressing